Having said that, you can eliminate this reliance around the client Pc's timezone and create a UTC date, a date with out a timezone. Discard the supernatant, resuspend cell pellets with two hundred μL one× D-PBS and Adhere to the exact process of extracellular staining with antibodies as explained in https://kaiserlichesinterieur.de/2024/04/29/entdecken-sie-die-kraft-der-liebe-6-moglichkeiten-mehr-liebe-in-ihrem-leben-zu-kultivieren/